PARCELLING FLOW RATE CONTROLLER FOR ELECTROPHORETIC APPARATUS

PURPOSE:To parcel components of equal charge accurately and efficiently, by arranging a parcelling hole at the tank thickness-wise center of a sample parcelling section to control the parcelling flow rate so that the speed of inflow from the parcelling hole is almost the same as that of a tank liqui...

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Bibliographische Detailangaben
Hauptverfasser: ASHIDA AKIRA, YAMAZAKI HARUYUKI, OHASHI HARUMICHI, TSUBOUCHI KUNIYOSHI, MATSUSHIMA TOKUNORI, MITANI KENJI
Format: Patent
Sprache:eng
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Zusammenfassung:PURPOSE:To parcel components of equal charge accurately and efficiently, by arranging a parcelling hole at the tank thickness-wise center of a sample parcelling section to control the parcelling flow rate so that the speed of inflow from the parcelling hole is almost the same as that of a tank liquid at an upstream point with respect to the parcelling hole. CONSTITUTION:A sample injection tube 2 and a tank liquid introduction tube 4 are provided on the top of a migration tank 1, electrodes 6 and 7 at both ends thereof, a sample parcelling section 3 at the lower part thereof and a tank liquid drain tube 5 on the bottom thereof. In addition, upper and lower detectors 16 and 17 are provided to detect absorbance with the wavelength allowing absorption by components to be separated. A diffusion range of the components is detected with the upper detector 16 from a detection range of absorbance at an upstream position with respect to a parcelling hole 15 while that is detected with the lower detector 17 from a detection range of absorbance at a position immediately before the parcelling hole. The amount of a parcelling liquid is controlled with a parcelling flow rate adjuster 24 to make the detection ranges equal thereby enabling the parcelling of components of equal charge accurately as well as efficiently.