E.COLI PLASMID CONTAINING INTRODUCED GROWTH HORMONE STRUCTURE GENE OF YELLOW-TAIL

PURPOSE:To enable the mass-production of yellow-tail growth hormone, by preparing an E.coli recombination plasmid amplifying a deoxynucleotide chain coding a yellow-tail pre-growth hormone containing yellow-tail growth hormone. CONSTITUTION:Pituitary gland of yellow-tail extracted from a yellow-tail...

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Hauptverfasser: YONEDA SUKEYASU, NAKAJIMA KUNIO
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NAKAJIMA KUNIO
description PURPOSE:To enable the mass-production of yellow-tail growth hormone, by preparing an E.coli recombination plasmid amplifying a deoxynucleotide chain coding a yellow-tail pre-growth hormone containing yellow-tail growth hormone. CONSTITUTION:Pituitary gland of yellow-tail extracted from a yellow-tail is disintegrated in a guanidium thiocyanate solution. An RNA fraction is collected and concentrated by centrifugal separation and bonded with an ampicillin- resistant gene of plasmid pSI4001 and a cloning primer. The obtained plasmid is introduced into a host E.coli DH1 strain and the strain is grown on an agar medium. The obtained amplifier plasmid of a yellow-tail pre-growth hormone structure gene has a duplex structure having a total length of 3,851 base pairs and containing a deoxynucleotide chain coding a yellow-tail growth hormone and an ampicillin resistant gene of pSI4001.
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subjects AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CARE OF BIRDS, FISHES, INSECTS
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DERIVATIVES THEREOF
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW BREEDS OF ANIMALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR
SPIRITS
SUGARS
TRAPPING
VINEGAR
WINE
title E.COLI PLASMID CONTAINING INTRODUCED GROWTH HORMONE STRUCTURE GENE OF YELLOW-TAIL
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