E.COLI PLASMID CONTAINING INTRODUCED GROWTH HORMONE STRUCTURE GENE OF YELLOW-TAIL

PURPOSE:To enable the mass-production of yellow-tail growth hormone, by preparing an E.coli recombination plasmid amplifying a deoxynucleotide chain coding a yellow-tail pre-growth hormone containing yellow-tail growth hormone. CONSTITUTION:Pituitary gland of yellow-tail extracted from a yellow-tail...

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Hauptverfasser:	YONEDA SUKEYASU, NAKAJIMA KUNIO
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS SUGARS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To enable the mass-production of yellow-tail growth hormone, by preparing an E.coli recombination plasmid amplifying a deoxynucleotide chain coding a yellow-tail pre-growth hormone containing yellow-tail growth hormone. CONSTITUTION:Pituitary gland of yellow-tail extracted from a yellow-tail is disintegrated in a guanidium thiocyanate solution. An RNA fraction is collected and concentrated by centrifugal separation and bonded with an ampicillin- resistant gene of plasmid pSI4001 and a cloning primer. The obtained plasmid is introduced into a host E.coli DH1 strain and the strain is grown on an agar medium. The obtained amplifier plasmid of a yellow-tail pre-growth hormone structure gene has a duplex structure having a total length of 3,851 base pairs and containing a deoxynucleotide chain coding a yellow-tail growth hormone and an ampicillin resistant gene of pSI4001.