HUMAN/HUMAN HYBRIDOMA TO PRODUCE HUMAN IMMUNO-GLOBULIN OF ANTIGENIC SPECIFICITY RELATED TO CANCER

NEW MATERIAL:A human immunoglobulin of antigen specificity produced by a human/human fusion cell strain of human B-cell of human stomach cancer patient and a subclone of a human lymphoblast cell strain. USE:A drug for preventing, remedying and diagnosing human lung cancer, brain tumor, malignant mel...

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Hauptverfasser: HAGIWARA HIDEAKI, AOZUKA YASUYUKI
Format: Patent
Sprache:eng
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Zusammenfassung:NEW MATERIAL:A human immunoglobulin of antigen specificity produced by a human/human fusion cell strain of human B-cell of human stomach cancer patient and a subclone of a human lymphoblast cell strain. USE:A drug for preventing, remedying and diagnosing human lung cancer, brain tumor, malignant melanoma, etc. PREPARATION:For example, a B-cell from a lymph node in the periphery of cancerous tissue collected in the operation of a stomach cancer patient and a subclone of a human lymphoblast cell strain are subjected to cell fusion in the presence of polyethylene glycol and cultivated in HAT medium, to give a hybridoma. Then, it is cloned by limiting dilution, etc., made into a monoclone, cultivated in a medium and a product is purified, to give a monoclonal antibody.