PROLACTIN POLYPEPTIDE DERIVATIVE OF FISH OR SUCH

PURPOSE:To obtain the titled compound useful as a growth promotor for fishes and an adjustor for fresh water adaptability, by cultivating a bacterium containing recombinant DNA integrated with DNA to code a prolactin polypeptide derivative of fish in a nutritive medium. CONSTITUTION:(A) A DNA fragme...

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Hauptverfasser:	KUGA TETSUO, ITOU SEIGA
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FODDER FOODS OR FOODSTUFFS HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To obtain the titled compound useful as a growth promotor for fishes and an adjustor for fresh water adaptability, by cultivating a bacterium containing recombinant DNA integrated with DNA to code a prolactin polypeptide derivative of fish in a nutritive medium. CONSTITUTION:(A) A DNA fragment containing DNA to code prolactin polypeptide of 1,200bp from plasmid pSPRL1 containing DNA to code prolactin polypeptide of salmon is combined with (B) a DNA fragment containing a terminator part of lpp system of 1,700bp from plasmid pKYP26 and (C) a DNA fragment containing trp type promotor of 1,000bp from plasmid pTrS20 to prepare recombinant DNA (plasmid pSPRLQ and pSPRLQ52). A bacterium (Escherichia coli is preferable) is transformed with the plasmid and the bacterium is cultivated in a medium to form the aimed polypeptide derivative in the culture mixture.