PLASMID USEFUL FOR PRODUCTION OF L-TRYPTOPHAN

PURPOSE:To obtain a recombinant plasmid having high gene amplification capability, by integrating two genetic information sets to regulate biosynthesis of tryptophan into one molecule of a plasmid DNA free from self-replicability in host microorganism. CONSTITUTION:The objective recombinant plasmid...

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Bibliographische Detailangaben
Hauptverfasser:	YAJIMA YOSHIHIRO, SAKIMOTO KAZUNORI, TAKAMATSU HISAO, TAKINISHI EIKO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To obtain a recombinant plasmid having high gene amplification capability, by integrating two genetic information sets to regulate biosynthesis of tryptophan into one molecule of a plasmid DNA free from self-replicability in host microorganism. CONSTITUTION:The objective recombinant plasmid contains two fragments of a chromosome DNA of a microorganism (preferably Bacillus amyloliquefaciens) having chloramphenicol-resistant gene and ampicillin-resistant gene and capable of producing L-tryptophan. Each of the fragments contains genetic information essentially participating in biosynthesis of L-tryptophan. The recombinant plasmid is e.g. pSDT391 shown in the figure. E.coli SD1011 containing the pSDT391 has been deposited in the Japanese Fermentation Research Institute as FERM P-8621.