PRODUCTION OF LIPASE

PURPOSE:To enhance productivity of lipase regardless of the kind of an organic nitrogen source, by adding a substrate consisting essentially of amino acid or amino acid and peptide to a culture medium and cultivating a microorganism while keeping the amino acid concentration in the culture fluid at a low value. CONSTITUTION:A substrate consisting essentially of an amino acid or amino acid and a peptide is added to a culture medium for a microorganism capable of producing lipase to carry out cultivation while keeping the amino acid concentration in the culture fluid at a low value. A microorganism belonging to the genus Rhizopus, Aspergillus or Mucor is used as the microorganisms capable of producing lipase. The substrate to be added contains preferably >=40%, more preferably >=60% amino acid or amino acid and peptide and the amount of a carbohydrate, saccharide, organic acid, etc., in the substrate to be a carbon source is