METHOD OF CONSTITUTING SHUTTLE VECTOR

PURPOSE:To obtain various kinds of transformed yeast, by integrating various genes into a shuttle vector carrying an inhibitory acidic phosphatase promotor besides a yeast gene and an Escherichia coli under control of the phosphatase promotor. CONSTITUTION:A recombinant plasmid carrying a yeast gene...

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Hauptverfasser: TOE AKIO, MIYANOHARA KOJI, MATSUBARA KENICHI
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creator TOE AKIO
MIYANOHARA KOJI
MATSUBARA KENICHI
description PURPOSE:To obtain various kinds of transformed yeast, by integrating various genes into a shuttle vector carrying an inhibitory acidic phosphatase promotor besides a yeast gene and an Escherichia coli under control of the phosphatase promotor. CONSTITUTION:A recombinant plasmid carrying a yeast gene, an Escherichia coli gene and an inhibitory acidic phosphatase phenotypic expression control range of yeast is treated with exonuclease BAL31, so structural gene of the acidic phosphate and part up to a given site of the structural gene are removed.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DERIVATIVES THEREOF
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
SUGARS
VINEGAR
WINE
title METHOD OF CONSTITUTING SHUTTLE VECTOR
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