PREPARATION OF ANTIBODY-FORMING CELLS

PURPOSE:The mixed cultivation of B lymphocytes and an antigen is carried out in vivo, the resultant antibody-producing cells are fused with tumor cells to form a hybridoma whereby cells producing a specific monoclonal antibody are prepared. CONSTITUTION:The spleen is removed from a mouce, ground to...

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Bibliographische Detailangaben
Hauptverfasser:	HOZUMI TOYOJI, IWATA HIKARI, SUZUKI HIDEKI, MORIYAMA MAYUMI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:The mixed cultivation of B lymphocytes and an antigen is carried out in vivo, the resultant antibody-producing cells are fused with tumor cells to form a hybridoma whereby cells producing a specific monoclonal antibody are prepared. CONSTITUTION:The spleen is removed from a mouce, ground to form a suspen sion, the suspension is filtered, centrifuged to recover B lumphocytes (A). The component A is mixed with an antigen such as nucleic acid or protein and the mixture is cultured in a medium in test tubes (such as RPM1-I640) in the presence of about 5% carbon dioxide at about 37 deg.C for 2-7 days. The resultant culture mixture is subjected to enzyme immunoassay to select the desired antibody-producing cells and the cells are fused with tumor cells.