METHOD FOR CULTIVATING B CELL

PURPOSE:To multiply efficiently B cells with improved cloning efficiency, by cultivating B cells transformed by EB viruses in a specific culture medium. CONSTITUTION:Lymphocytes obtained by separating from human peripheral blood holding a specific antibody are transformed by EB viruses to give B cel...

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Bibliographische Detailangaben
Hauptverfasser:	UNO MASATSUNE, NAGATSUKA YASUKO, YAMADA SUGURU, ONO ISAO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To multiply efficiently B cells with improved cloning efficiency, by cultivating B cells transformed by EB viruses in a specific culture medium. CONSTITUTION:Lymphocytes obtained by separating from human peripheral blood holding a specific antibody are transformed by EB viruses to give B cells, which are cultivated in a culture medium prepared by irradiating tumorigenic transformed B cell derived from Burkitt's lymphoma, e.g. Raji, Daudi or BJA-B cell, or adding B cell multiplication factor produced from the B cell as a feeder cell in 300,000-500,000 number of cells per one plate (6cm) to RPMI1640 (containing added 20% bovine fetal blood serum), etc., by the limiting dilution method, etc.