NOVEL HYBRIDOMA
PURPOSE:To obtain a novel hybridoma useful as a parent strain for cell fusion, having high fusion efficiency with an antibody-forming cell, by fusing a human lymphocyte cell with a cell strain like lymphocyte of different animal. CONSTITUTION:A human lymphocyte cell (normal lymphocyte obtained from...
Gespeichert in:
Hauptverfasser: | , , |
---|---|
Format: | Patent |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | PURPOSE:To obtain a novel hybridoma useful as a parent strain for cell fusion, having high fusion efficiency with an antibody-forming cell, by fusing a human lymphocyte cell with a cell strain like lymphocyte of different animal. CONSTITUTION:A human lymphocyte cell (normal lymphocyte obtained from healthy human or human established lymphocyte cell, etc.) is fused with a cell strain like lymphocyte of different animal (mouse myeloma cell strain, etc.) by using a fusing agent such as sendai virus, polyethylene glycol, etc., or by electrical stimulation method, etc. The prepared hetero hybridoma and a human lyophocyte cell to form a useful antibody are subjected to cell fusion to produce a cloned hybridoma and a useful monoclonal antibody, and the prepared hetero hybridoma is safely handled. |
---|