NOVEL HYBRIDOMA

PURPOSE:To obtain a novel hybridoma useful as a parent strain for cell fusion, having high fusion efficiency with an antibody-forming cell, by fusing a human lymphocyte cell with a cell strain like lymphocyte of different animal. CONSTITUTION:A human lymphocyte cell (normal lymphocyte obtained from...

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Bibliographische Detailangaben
Hauptverfasser: TSUKAMOTO KYOZO, SASANO KAZUNORI, ICHIMORI YUUZOU
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:PURPOSE:To obtain a novel hybridoma useful as a parent strain for cell fusion, having high fusion efficiency with an antibody-forming cell, by fusing a human lymphocyte cell with a cell strain like lymphocyte of different animal. CONSTITUTION:A human lymphocyte cell (normal lymphocyte obtained from healthy human or human established lymphocyte cell, etc.) is fused with a cell strain like lymphocyte of different animal (mouse myeloma cell strain, etc.) by using a fusing agent such as sendai virus, polyethylene glycol, etc., or by electrical stimulation method, etc. The prepared hetero hybridoma and a human lyophocyte cell to form a useful antibody are subjected to cell fusion to produce a cloned hybridoma and a useful monoclonal antibody, and the prepared hetero hybridoma is safely handled.