NOVEL HYBRIDOMA, PREPARATION AND USE THEREOF

PURPOSE:To obtain a hybridoma capable of producing high-purity antibody in high efficiency, by fusing a lymphocyte hybridoma of human and heteroanimal with a human lymphocyte capable of producing a specific antibody, selecting the desired hybridoma from the fused cell, and cloning the hybridoma. CON...

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Hauptverfasser:	TSUKAMOTO KYOZO, SASANO KAZUNORI, ICHIMORI YUUZOU
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To obtain a hybridoma capable of producing high-purity antibody in high efficiency, by fusing a lymphocyte hybridoma of human and heteroanimal with a human lymphocyte capable of producing a specific antibody, selecting the desired hybridoma from the fused cell, and cloning the hybridoma. CONSTITUTION:Activated human lymphocyte (e.g. peripheral blood lymphocyte) and a lymphoidocyte strain of a heteroanimal (e.g. mouse) are fused together by treating in a medium containing 10-80wt% polyethylene glycol having a polymerization degree of 1,000-6,000 for 0.5-30min. The fused cell is cultured in a selective medium added with 8-azaguanine, etc., to obtain a hybridoma cell (heterohybridoma) introduced with a marker. Separately, a human lymphocyte infected with hepatitis B virus and positive to the anti-hepatitis B virus surface antigen antibody is separated in vitro, stimulated with an antigen, and is fused with the above cell. The obtained fused cell is proliferated in a selective medium. The antibody value of the supernatant liquid is determined, and the cell positive to the test is selected.