METHOD FOR MEASURING SUBSTRATE OR ENZYME

PURPOSE:To make it possible to carry out automatic analysis of a substrate or enzyme, by leading reduced form of nicotinamide adenine dinucleotide (NADH) or reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) produced in the dehydrogenase reaction through an electron carrier to the coloration with hydrogen peroxide. CONSTITUTION:In a method for measuring a substrate or enzyme through reduced form of nicotinamide adenine dinucleotide (NADH) or nicotinamide adenine dinucleotide phosphate (NADPH), an electron carrier, e.g. phenazine methosulfate (PMS), 1-methoxyphenazine methosulfate (1-methoxyPMS) or phenazine ethosulfate (PES), is present therein. These electron carriers capable of producing H2O2 when the reduced form thereof is returned to the original oxidized form by oxygen dissolved in the reaction solution. The H2O2 is colored by the well-known enzymic or nonenzymic method to measure the substrate or enzyme. According to this method, the automation of the measurement is easily carried out.