MANUFACTURE OF ENZYME SENSOR FOR MEASURING LIPID

PURPOSE:To enable the manufacture of an enzyme sensor for measuring lipid handily while facilitating miniaturization by arranging two hydrogen ion responsive FET type transistor and a reference electrode so that a photosensitive resin containing lipase is applied and hardened on an ion responsive su...

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Bibliographische Detailangaben
Hauptverfasser:	HANASATO YOSHIO, NAKAKO MAMIKO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To enable the manufacture of an enzyme sensor for measuring lipid handily while facilitating miniaturization by arranging two hydrogen ion responsive FET type transistor and a reference electrode so that a photosensitive resin containing lipase is applied and hardened on an ion responsive surface of one transistor thereof to form a lipase immobilized film. CONSTITUTION:A lipase immobilized film is formed on a part of an ion responsive surface 8 of pH-ISFET (A) and none thereof is formed on the other pH- ISFET (B). With such an arrangement, an enzyme sensor for measuring lipid is manufactured. Any lipid in a sample solution is decomposed and pH in the lipase immobilized film develops a difference in the pH of the sample solution itself monitored by the pH-ISFET (B) without any lipase immobilized film. Thus, the enzyme sensor for measuring lipid measures the source voltage necessary for running a fixed current between the sources and drains of the two pH-ISFETs (A) and (B) and amplifies the differential outputs of the source voltages of both the pH-ISFETs thereby determining the concentration of lipid in the sample solution.