ACTINOMYCES COSMID VECTOR

ACTINOMYCES COSMID VECTOR

PURPOSE:To insert a vector into the microbial cells of actinomyces without utilizing protoplast method, by using a cosmid vector having a cohesive site derived from an actinomyces phage inserted into a plasmid derived from actinomyces. CONSTITUTION:An actinomyces cosmid vector, obtained by inserting...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: TAKAHASHI HIDEO, SAITOU AKIHISA, MORINO TOMIO
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:PURPOSE:To insert a vector into the microbial cells of actinomyces without utilizing protoplast method, by using a cosmid vector having a cohesive site derived from an actinomyces phage inserted into a plasmid derived from actinomyces. CONSTITUTION:An actinomyces cosmid vector, obtained by inserting a cohesive site derived from actinomyces phage into a plasmid derived from actinomyces, and having a cohesive site COS derived from the actinomyces phage and one or more cloning sites (kpnI, XbaI or ScaI, etc.). pR4C-1 (FERM-P No.7204), etc. may be cited as examples of the cosmid. The cosmid is obtained by inserting the cohesive site of actinomyces phage R4 into a ClaI restriction enzyme site of a plasmid pIJ365 derived from the actinomyces.