ESCHERICHIA COLI

PURPOSE:To obtain Escherichia coli capable of producing novel heat-resistant L- alanine dehydrogenase, by transforming Escherichia coli with a plasmid having the gene of a heat-resistant L-alanine dehydrogenase. CONSTITUTION:The gene of an L-alanine dehydrogenase originated from the chromosome DNA o...

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Bibliographische Detailangaben
Hauptverfasser:	OOSHIMA TOSHIHISA, SAKAMOTO YONEKAZU, NAGATA SHINJI, TANAKA HIDEHIKO, SOUDA KENJI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To obtain Escherichia coli capable of producing novel heat-resistant L- alanine dehydrogenase, by transforming Escherichia coli with a plasmid having the gene of a heat-resistant L-alanine dehydrogenase. CONSTITUTION:The gene of an L-alanine dehydrogenase originated from the chromosome DNA of Bacillus stearothermophilus is introduced into plasmid pBR322 to obtain plasmid pICR3 and pICR301. These plasmids are introduced into an Escherichia coli to obtain Escherichia coli C600-pICR (FERM-P No.7447) and Escherichia coli C600-pICR301 (FERM-P No.7448). A heat-resistant L-alanine dehydrogenase can be produced by culturing these bacterial strains.