DETECTION OF AMOUNT OF BACTERIA IN AIR

PURPOSE:To detect an amount of bacteria in air in a real time rapidly, by passing air to be tested through an extracting solution for a given time to extract ATP (adenosinemyrinic acid), adding a coloring reagent to it, detecting luminous energy of emission electrically. CONSTITUTION:The extracting solution 100 consisting of tris EDTA buffer solution is sent to the extraction device 10 consisting of the bubbling tube 12 and the collecting well 14, it is made into a boiling state, air in the chamber 102 is sucked by the pump 24, the sucked air is brought into contact with the extracting solution sufficiently, and ATP is extracted from bacteria contained in air. Impurities are removed from the solution having extracted ATP by the filter 32, cooled by the cooler 34, sent to the detection cell 30. A given amount of a coloring reagent (e.g., luciferin, or luciferase) 104 is added to it, emission of an amount of ATP, namely emission proportional to an amount of bacteria is caused, the emission is measured electrically by the photoelectron amplifying tube 40, and the amount of bacteria is outputted as a digital value by the calculator 42 for luminous energy.