MOTHER CELL STRAIN FOR PREPARATION OF HUMAN HYBRIDOMA

NEW MATERIAL:The mother cell strain for the preparation of human hybridoma which is a hypoxanthine-guanine-phosphoribosyltransferase-dificient mutant of a Namalwa cell which is a kind of human Burkitt's lymphoma cell. USE:A mother cell strain for the preparation of human hybridoma having excell...

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Hauptverfasser:	MURAKAMI HIRONORI, HASHIZUME SHIYUUICHI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Zusammenfassung:	NEW MATERIAL:The mother cell strain for the preparation of human hybridoma which is a hypoxanthine-guanine-phosphoribosyltransferase-dificient mutant of a Namalwa cell which is a kind of human Burkitt's lymphoma cell. USE:A mother cell strain for the preparation of human hybridoma having excellent fusion capability to human lymphocyte B and capable of producing an antibody. PREPARATION:The cell is cultured in a serum medium added with preferably 30mug/ml of 6-thioguanine, at 37 deg.C in a 5% CO2 gas incubator, refreshing the medium every 3-5 days.