MIXED ACTIVE SUBSTANCE FOR ENHANCING SECRETION OF INSULIN AND PRODUCTION THEREOF

PURPOSE:To obtain the titled active substance from a specific proteinic subunit substance and a proteinic modifying subunit substance. CONSTITUTION:A mixed active substance (IAP) for enhancing the secretion of insulin obtained from at least one of a proteinic subunit, e.g. Ps-1, P-3, P-4 or Ps- 5, constituting an active substance for enhancing the secretion of insulin produced by a microorganism of the genus Bordetella and a proteinic modifying subunit substance which is a modifier of a given proteinic subunit substance. The above-mentioned subunit substance is obtained by reacting an active substance for enhancing the secretion of insulin produced by a microorganism of the genus Bordetella with an amidination agent of the formula (R1 is H, 1-4C lower alkyl or chloromethyl, etc.; R2 is 1-4C lower alkyl), and macerating the resultant compound. The above-mentioned mixed IAP has weakened leukocytotic action though with an effective pharmacological activity almost the same as that of the unmodified IAP.