PREPARATION OF THERMOPHILIC AMYLASE

PREPARATION OF THERMOPHILIC AMYLASE

PURPOSE:To prepare a thermophilic amylase, in high yield, by culturing a novel microbial strain belonging to Escherichia genus and capable of producing thermophilic amylase. CONSTITUTION:A DNA fragment separated from the chromosome DNA of Bacillus thermophilus, having a molecular weight of 2.9X10 kb...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: IHARA ISATO, YAMAGATA HIDEO, TSUKAGOSHI NORIHIRO, UDAKA JIYUUZOU
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:PURPOSE:To prepare a thermophilic amylase, in high yield, by culturing a novel microbial strain belonging to Escherichia genus and capable of producing thermophilic amylase. CONSTITUTION:A DNA fragment separated from the chromosome DNA of Bacillus thermophilus, having a molecular weight of 2.9X10 kbp, coding thermopholic amylase protein, and having a scission site with BamHI in the DNA chain, a scission site with SalI 2.4X10 bp downstream of the above site, a scission site with PstI further 0.5X10 bp downstream, and a scission site with BamHI further 1.4X10 bp downstream, is integrated in a plasmid vector, and is introduced into a host microorganism. The obtained microorganism belonging to Escherichia genus, e.g. Escherichia coli HB101/pHI301 (FERM-P No.7009) is cultured, and the objective thermophilic amylase is collected mainly from the cultured microbial cells.