PERTUSSIS VACCINE CONTAINING B-OLIGOMER AND ITS PREPARATION

PERTUSSIS VACCINE CONTAINING B-OLIGOMER AND ITS PREPARATION

PURPOSE:To provide the titled vaccine having high activity and stability, by using a specific B-oligomer as an active component. CONSTITUTION:A pertussis vaccine is prepared by using the B-oligomer (molecular weight, 75,000+ or -5,000 by SDS-gel electrophoresis; protein content, >=98wt% by Lowry...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: ASE KATSUHIKO, TAKAHASHI ISAMU, TAMURA MAKOTO, ITOU KIYOSHI, YAJIMA MOTOYUKI, TOMIOKA CHIKANORI, SATOU SOUICHIROU
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROCESSES USING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:PURPOSE:To provide the titled vaccine having high activity and stability, by using a specific B-oligomer as an active component. CONSTITUTION:A pertussis vaccine is prepared by using the B-oligomer (molecular weight, 75,000+ or -5,000 by SDS-gel electrophoresis; protein content, >=98wt% by Lowry process; amino acid composition and composition ratios (muM/100muM) of the protein component, as shown in the table I ; basic protein) as an active component. The B-oligomer can be prepared by (1) culturing microorganisms belonging to Bordetella genus (e.g. Bordetella pertussis phase I ), (2) treating the supernatant liquid of the cultured liquid with hydroxyapatite to obtain crude fraction containing insulin-secretion promoting factor, (3) adding urea to the crude fraction to an ultimate urea concentration of 3-6M, (4) incubating the mixture, (5) treating with haptoglobin sepharose, and (6) purifying the product.