PURIFICATION OF INTERFERON

PURPOSE:To recover interferon, in high yield, by contacting an interferon with an inorganic adsorbent in a highly concentrated salt solution, and eluting the adsorbed interferon with an eluent. CONSTITUTION:An interferon is made to contact with an inorganic adsorbent in a highly concentrated salt so...

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Bibliographische Detailangaben
Hauptverfasser:	TAKAZAWA SEIGO, YOKOO YOSHIHARU, HASHIMOTO YUKIO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To recover interferon, in high yield, by contacting an interferon with an inorganic adsorbent in a highly concentrated salt solution, and eluting the adsorbed interferon with an eluent. CONSTITUTION:An interferon is made to contact with an inorganic adsorbent in a highly concentrated salt solution to effect the adsorption of the interferon to the adsorbent. The adsorbed interferon is eluted with an eluent. The interferon is, e.g. originated from interferon-producing microorganisms, human fibroblast cells, mouse cells, etc., and the salt in the solution is sulfate, chloride, phosphate, etc. The salt concentration is >=0.05M, preferably 0.5-1.5M. Too high concentration of the salt results in the precipitation of the interferon by salting out. The pH of the solution is 5-8, preferably 6.5-7.5.