ENZYMATIC DYEING METHOD FOR SEPARATED PROTEIN SPOTS

PURPOSE:Proteins are electrophoretically separated in a support and hemin is adsorbed to these proteins, then dyed with a pigment precursor and hydrogen peroxide to give an optical contrast against the parts where proteins are not adsorbed, thus conducting the determination of proteins or the like....

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Bibliographische Detailangaben
Hauptverfasser:	ITOU MICHIO, YOSHIDA MOTOKO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:Proteins are electrophoretically separated in a support and hemin is adsorbed to these proteins, then dyed with a pigment precursor and hydrogen peroxide to give an optical contrast against the parts where proteins are not adsorbed, thus conducting the determination of proteins or the like. CONSTITUTION:A support containing proteins separated by electrophoresis is dipped in a buffer aqueous solution containing hemin to cause the adsorption of hemin to the proteins. The resultant support is dipped in a buffer-aqueous solution containing no hemin to remove the hemin existing in the protein-free parts. Then, the support is dipped in a buffer aqueous solution containing 2,4-dibromo- 1-naphthol and N,N-dimethyl-p-phenylenediamine or another pigment precursor, then hydrogen peroxide is added, resultingly a pigment is synthesyzed on the separated protein molecules to develop a color.