JPS5825980B

An assay kit for assaying adenosine-3',5'-cyclic monophosphate, which is herein referred to as cAMP, guanosine-3',5'-cyclic monophosphate, which is herein referred to as a cGMP, or both; and said assay kit comprising, A CONTAINER OF Reagent 1 comprising succinyl cAMP tyrosine methyl ester; succinyl cGMP tyrosine methyl ester; succinyl cAMP tyrosine methyl ester and succinyl cGMP tyrosine methyl ester in admixture or both succinyl cAMP tyrosine methyl ester and succinyl cGMP tyrosine methyl ester in separate containers, said esters labelled with radioactive iodine; A CONTAINER OF Reagent 2 comprising an anti-cAMP serum, an anti-cGMP serum, an anti-cAMP and anti-cGMP serum in admixture or both anti-cAMP serum and anti-cGMP serum in separate containers; A CONTAINER OF Reagent 3 comprising an imidazole buffer solution of 0.1 M or higher concentration; A CONTAINER OF Reagent 4 comprising a material for separation; A CONTAINER OF Reagent 5 comprising a solution of succinic anhydride in an organic solvent; A CONTAINER OF Reagent 6 comprising an organic tertiary amine; and A CONTAINER OF Reagent 7 comprising a standard solution of cAMP, cGMP, cAMP and cGMP in admixture or both cAMP and cGMP in separate containers.