ESTIMATION OF CALCITONIN

PURPOSE:To achieve a highly sensitive estimation of a calcitonin with a simple operation by a method wherein a sample calcitonin and an enzyme labelled calcitonin are brought to an immunological reaction with a calcitonin antibody competitively to form a composite antibody, which is bonded to a column filled with a second antibody non-soluble carrier to measure the activity of the enzyme. CONSTITUTION:A dilution liquid of a calcitonin antibody is added to a butacalcitonin standard liquid and a beta-galactosidase labelled calcitionin to the mixture to react. Then, the reaction liquid flows to a column filled with the second antibody non-soluble carrier. Then, after the column is washed with a buffer liquid G twice, a O-nitrophenyl-beta-D galactopyranoside solution flows to the column. After the reaction, 0.08M sodium carbonate flows twice to the column to wash. Then, the absorbance is measured for the 420nm of the washing liquid. As a result, a calibration curve as illustrated will be obtained.