PROTEIN ELEMENT SUBSTANCE AND ITS PREPARATION

NEW MATERIAL:Protein element substance CP-A. A molecular weight by SDS-gel electrophoresis being 75,000+ or -5,000. Protein by Lowry method of >=98wt%. Amino composition and composition ratio (muM/100muM) of protein component: Asp. 6.4+ or -0.7, Thr. 7.3+ or -0.8, Ser. 8.2+ or -0.9, Glu. 9.3+ or...

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Hauptverfasser: MURAI TOSHI, TAMURA MAKOTO, ITOU KIYOSHI, TOMIOKA CHIKANORI
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creator MURAI TOSHI
TAMURA MAKOTO
ITOU KIYOSHI
TOMIOKA CHIKANORI
description NEW MATERIAL:Protein element substance CP-A. A molecular weight by SDS-gel electrophoresis being 75,000+ or -5,000. Protein by Lowry method of >=98wt%. Amino composition and composition ratio (muM/100muM) of protein component: Asp. 6.4+ or -0.7, Thr. 7.3+ or -0.8, Ser. 8.2+ or -0.9, Glu. 9.3+ or -1.0, Pro. 6.3+ or -0.7, Gly. 11.1+ or -1.0, Ala. 9.0+ or -0.9, Cys./2 1.2+ or -0.3, Val. 6.2+ or -0.6, Met. 2.7+ or -0.4, Ile. 3.4+ or -0.4, Leu. 8.4+ or -0.9, Tyr. 5.6+ or -0.6, Phe. 3.6+ or -0.5, Lys. 4.7+ or -0.5, His. 1.1+ or -0.3, Arg. 4.9+ or -0.5. Basic protein. USE:A constructional component of active element of insulin secretion enhancement, and a remedy for diabetes having no side effects. PROCESS:A bacterium belonging to the genus Bordetella (e.g., Bortella pertussis, etc.) is cultivated and purified to give an active substance of insulin secretion enhancement, which is incubated by a 3-5M urea-containing phosphate buffer solution and separated and purified, to give protein element substance CP-A.
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A molecular weight by SDS-gel electrophoresis being 75,000+ or -5,000. Protein by Lowry method of &gt;=98wt%. Amino composition and composition ratio (muM/100muM) of protein component: Asp. 6.4+ or -0.7, Thr. 7.3+ or -0.8, Ser. 8.2+ or -0.9, Glu. 9.3+ or -1.0, Pro. 6.3+ or -0.7, Gly. 11.1+ or -1.0, Ala. 9.0+ or -0.9, Cys./2 1.2+ or -0.3, Val. 6.2+ or -0.6, Met. 2.7+ or -0.4, Ile. 3.4+ or -0.4, Leu. 8.4+ or -0.9, Tyr. 5.6+ or -0.6, Phe. 3.6+ or -0.5, Lys. 4.7+ or -0.5, His. 1.1+ or -0.3, Arg. 4.9+ or -0.5. Basic protein. USE:A constructional component of active element of insulin secretion enhancement, and a remedy for diabetes having no side effects. PROCESS:A bacterium belonging to the genus Bordetella (e.g., Bortella pertussis, etc.) is cultivated and purified to give an active substance of insulin secretion enhancement, which is incubated by a 3-5M urea-containing phosphate buffer solution and separated and purified, to give protein element substance CP-A.</abstract><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROCESSES USING MICROORGANISMS
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
SPIRITS
VINEGAR
WINE
title PROTEIN ELEMENT SUBSTANCE AND ITS PREPARATION
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