PROTEIN ELEMENT SUBSTANCE AND ITS PREPARATION

NEW MATERIAL:Protein element substance CP-A. A molecular weight by SDS-gel electrophoresis being 75,000+ or -5,000. Protein by Lowry method of >=98wt%. Amino composition and composition ratio (muM/100muM) of protein component: Asp. 6.4+ or -0.7, Thr. 7.3+ or -0.8, Ser. 8.2+ or -0.9, Glu. 9.3+ or...

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Bibliographische Detailangaben
Hauptverfasser:	MURAI TOSHI, TAMURA MAKOTO, ITOU KIYOSHI, TOMIOKA CHIKANORI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	NEW MATERIAL:Protein element substance CP-A. A molecular weight by SDS-gel electrophoresis being 75,000+ or -5,000. Protein by Lowry method of >=98wt%. Amino composition and composition ratio (muM/100muM) of protein component: Asp. 6.4+ or -0.7, Thr. 7.3+ or -0.8, Ser. 8.2+ or -0.9, Glu. 9.3+ or -1.0, Pro. 6.3+ or -0.7, Gly. 11.1+ or -1.0, Ala. 9.0+ or -0.9, Cys./2 1.2+ or -0.3, Val. 6.2+ or -0.6, Met. 2.7+ or -0.4, Ile. 3.4+ or -0.4, Leu. 8.4+ or -0.9, Tyr. 5.6+ or -0.6, Phe. 3.6+ or -0.5, Lys. 4.7+ or -0.5, His. 1.1+ or -0.3, Arg. 4.9+ or -0.5. Basic protein. USE:A constructional component of active element of insulin secretion enhancement, and a remedy for diabetes having no side effects. PROCESS:A bacterium belonging to the genus Bordetella (e.g., Bortella pertussis, etc.) is cultivated and purified to give an active substance of insulin secretion enhancement, which is incubated by a 3-5M urea-containing phosphate buffer solution and separated and purified, to give protein element substance CP-A.