MEASURING METHOD OF FREE FATTY ACID

PURPOSE:To determine acyl coenzyme A (acyl CoA), etc. and avoid the measurement interference by the clouding of a blood serum, by reacting an acyl CoA synthetase with a sample containing a free fatty acid, CoA, adenosine-5'-triphosphate (ATP) and lipoprotein, and inactivating the CoA, ATP or ac...

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Bibliographische Detailangaben
Hauptverfasser:	ARAO SHINSUKE, TSUBOTA HIROYUKI, TAKIZAWA NORIMASA, FUNAZAKI SACHIKO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To determine acyl coenzyme A (acyl CoA), etc. and avoid the measurement interference by the clouding of a blood serum, by reacting an acyl CoA synthetase with a sample containing a free fatty acid, CoA, adenosine-5'-triphosphate (ATP) and lipoprotein, and inactivating the CoA, ATP or acyl CoA synthetase, and reacting a lipoproteinic lipase with the resultant sample. CONSTITUTION:An acyl coenzyme A synthetase is reacted with a sample e.g. a blood serum containing a free fatty acid, coenzyme A (CoA), adenosine-5'-triphosphate (ATP) and lipoprotein, to produce an acyl coenzyme A, adenosine-5'- monophosphate and pyrophosphoric acid. The CoA, ATP or acyl coenzyme A synthetase remaining in the reaction solution is then inactivated with an SH reagent, an enzyme capable of decomposing the ATP, a chelating agent, and a lipoproteinic lipase is then reacted with the reaction solution. The measurement is carried out by determining the acyl CoA or adenosine-5'-monophosphate corresponding to the amount of the free acid in the sample stoichiometrically.