ANTIBODY AGAINST HEPATOCELL GROWTH FACTOR AND HEPATOCELL GROWTH FACTOR MEASURING METHOD

PROBLEM TO BE SOLVED: To provide a highly sensitive immunological measuring method of hepatocell growth factor(HGF), and an antibody used therefor and a preparing method for the antibody by obtaining the antibody linked to HGF from the vitellus of oviposited egg of a bird after charging HGF to the bird as the immunogen. SOLUTION: HGF is charged to a bird together with an adjuvant. For the inoculation a hypodermic injection is desirable. For the introjection of antibody per one injection, 0.01-10 mg or so is desirable. Since the oviposition of a specific antibody is made in the vitellus of egg of bird within a few weeks, the vitellus is separated from the egg subjected to the immunity, and lipoprotain and lipid are removed therefrom. The removement is subjected to treatment of organic solvent such as diethylether. The anti-HGF antibody value of the obtained solution is measured and one having a higher antibody value is selected. Saturated ammonium sulfate solution is added to this delipoprotein-delipid solution to perform salting-out sedimentation. Otherwise polymer such as polyethylene glycole is added thereto to coaggulate the antibody. Then, a centrifugal process is applied thereto and the antibody is dissolved with plysiological phosphoric buffering solution. Accordingly, a roughly prepared antibody is obtained.