CULTURE OF MICROBIAL CELL

PROBLEM TO BE SOLVED: To provide a method for efficiently culturing Pseudomonas bacteria having the ability to stably degrade TCE. SOLUTION: This method comprises culturing Pseudomonas bacteria having the ability to degrade trichloroethylene (TCE); specifically, the method is as follows: Pseudomonas...

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Bibliographische Detailangaben
Hauptverfasser:	OSAWA TAKEHIKO, KITAGAWA MASAYASU, HIROSE AKIRA
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DISPOSAL OF SOLID WASTE ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECLAMATION OF CONTAMINATED SOIL RECLAMATION OF CONTAMINED SOIL SOIL SPIRITS TRANSPORTING TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method for efficiently culturing Pseudomonas bacteria having the ability to stably degrade TCE. SOLUTION: This method comprises culturing Pseudomonas bacteria having the ability to degrade trichloroethylene (TCE); specifically, the method is as follows: Pseudomonas bacteria is subjected to preculture at 10-40 deg.C using a culture fluid at pH 5-9 with an initial toluene concentration of 100-400 mg/L containing at least a phosphorus source, nitrogen source, calcium, magnesium and iron; wherein it is preferable that the preculture is carried out by repeating batch cultures twice to seven times.