IMMOBILIZATION OF NUCLEIC ACID AND DETECTION OF NUCLEIC ACID USING THE SAME

PROBLEM TO BE SOLVED: To provide a method for detecting a nucleic acid in high sensitivity in excellent operability, by reacting the free functional group B of a magnetic particle of a specific polyfunctional group wish a nucleic acid. SOLUTION: A magnetic particle containing a functional group A su...

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Bibliographische Detailangaben
Hauptverfasser:	KOSUGE JUNICHI, NOZAWA MASAYUKI, IZUMI ATSUSHI
Format:	Patent
Sprache:	eng
Schlagworte:	ADHESIVES BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DYES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS MUTATION OR GENETIC ENGINEERING NATURAL RESINS PAINTS PHYSICS POLISHES PREPARATION OF CARBON BLACK PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING TREATMENT OF INORGANIC MATERIALS, OTHER THAN FIBROUS FILLERS,TO ENHANCE THEIR PIGMENTING OR FILLING PROPERTIES VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method for detecting a nucleic acid in high sensitivity in excellent operability, by reacting the free functional group B of a magnetic particle of a specific polyfunctional group wish a nucleic acid. SOLUTION: A magnetic particle containing a functional group A such as amino group, carboxyl group or the like is bound with a polyfunctional polymer containing >=3 functional groups to be reacted with the functional group A, such as a polyacidic amino acid, a polybasic amino acid or the like by the reaction between the functional groups A and B to prepare a polyfunctional magnesium particle and then its free functional group B is reacted with a nucleic acid to immobilize the nucleic acid. Preferably a nucleic acid is determined by taking advantage of the method for immobilizing a nucleic acid.