PRODUCTION OF PROTEIN

PROBLEM TO BE SOLVED: To inexpensively produce a protein such as a foreign protein by culturing a (non)transformed eukaryocyte under conditions using an inhibitory substrate as a carbon source and restricting the carbon source during a feeding phase and not causing a continuous oxygen restriction du...

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Bibliographische Detailangaben
Hauptverfasser:	LOPEZ-ULIBARRI RUAL, HELLMUTH KARSTEN, SCHLIEKER HEINRICH WINFRIED, MAYER ANNE FRANCOISE, VAN LOON ADOLPHUS
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To inexpensively produce a protein such as a foreign protein by culturing a (non)transformed eukaryocyte under conditions using an inhibitory substrate as a carbon source and restricting the carbon source during a feeding phase and not causing a continuous oxygen restriction during the culture. SOLUTION: This method for producing a protein such as a foreign or different phytase, cellulase, xylanase or industrial enzyme comprises culturing a transformed or non-transformed eukaryocyte under conditions using an inhibitory substrate as a carbon source, restricting the carbon source during a feeding phase and not generating a continuous oxygen restriction during the total period of the culture. It is preferable that the cell is transformed with a DNA sequence containing methylotroph and/or the promoter of an enzyme related to the metabolism of methanol, such as formic acid dehydrogenase promoter, methanol oxidase promoter or dihydroxyacetone synthase.