USE OF WASHING WITH ALCOHOL-SALT BUFFER FOR EFFICIENT RECOVERY OF MYCOBACTERIUM AND MYCOBACTERIUM DNA FROM RESPIRATORY SEDIMENTATION

PROBLEM TO BE SOLVED: To efficiently concentrate the bacterium cells and use them for nucleic acid analysis by adding a specified density-lowering agent to a viscous biological sample in a sufficient amount to reduce the density of the viscous sample lower than the density of the bacterium cells, ce...

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Bibliographische Detailangaben
Hauptverfasser:	MEHTA RASHMI, BACKUS JOHN W, KERSCHNER JOANNE H, KREADER CAROL
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To efficiently concentrate the bacterium cells and use them for nucleic acid analysis by adding a specified density-lowering agent to a viscous biological sample in a sufficient amount to reduce the density of the viscous sample lower than the density of the bacterium cells, centrifuging and pelletizing the bacterium cells. SOLUTION: A viscous biological sample selected from the group consisting of respiratory samples, respiratory sedimentation, oral cavity juice, vaginal secretion, semen, wound infection liquid and abscess liquid is diluted with a water-soluble density-lowering agent of a (substituted) methyl alcohol or (substituted) propyl alcohol in a sufficient amount to reduce the density of the sample lower than that of the bacterium cells having 0.7-0.9 g/ml density and boiling at a temp. higher than 50 deg.C, centrifuging and pelletizing arbitrary cells in the sample thereby concentrating the bacterium cells. In a preferred embodiment, the viscous biological sample is pretreated with a reagent that inhibits the analysis of nucleic acid.