RECOMBINANT-TYPE ANTI-HUMAN TNF-ALPHA HUMAN MONOCLONAL ANTIBODY

PROBLEM TO BE SOLVED: To produce the subject antibody having high purity suitable for an experimental reagent or a clinical application by applying a gene recombination technique by using a host Escherichia coli. SOLUTION: This recombinant-type anti-human TNF-α human monoclonal antibody is composed...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	IHARA SEIJI, TAKEKOSHI MASATAKA, TAKEKOSHI FUMIKO, ONO ISAO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To produce the subject antibody having high purity suitable for an experimental reagent or a clinical application by applying a gene recombination technique by using a host Escherichia coli. SOLUTION: This recombinant-type anti-human TNF-α human monoclonal antibody is composed of an H chain having an amino acid sequence of formula I and acting as an H chain of anti-human TNF-α human monoclonal Fab antibody and an L chain having an amino acid sequence of formula II and acting as an L chain of anti-human TNF-α human monoclonal Fab antibody. The recombinant-type anti-human TNF-α human monoclonal antibody is obtained by selecting and collecting a cDNA of the human antibody from a human B lymphocytes cell producing an anti-human TNF-αhuman antibody such as an anti-human TNF-α human monoclonal antibody producing 1D5 strain (EBV transformed B lymphocytes oligo clone), introducing the cDNA into a manifestation vector producing a human Fab antibody in an Escherichia coli by a gene recombination and culturing its transforming bacterium.