CLONING AND PRODUCTION OF RESTRICTION ENDONUCLEASE SAPI IN ESCHERICHIA COLI

PROBLEM TO BE SOLVED: To obtain a new isolated DNA originated from a Saccharopolyspora species, coding SapI restriction endonuclease and useful for producing a restriction enzyme capable of cleaving a DNA molecule into precise fragments, etc. SOLUTION: This new isolated DNA is obtained from Saccharo...

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Hauptverfasser:	XU SHUANG-YONG, XIAO JIAN-PING, MAUNUS ROBERT E
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To obtain a new isolated DNA originated from a Saccharopolyspora species, coding SapI restriction endonuclease and useful for producing a restriction enzyme capable of cleaving a DNA molecule into precise fragments, etc. SOLUTION: This new isolated DNA is obtained from Saccharopolyspora species and codes SapI restriction endonuclease. The isolated DNA is useful for producing by a genetic recombination method the restriction endonuclease capable of cleaving a DNA molecule into precise fragments for the cloning of the molecule and the characterization of the gene, etc. The isolated DNA is obtained by separating a genom DNA from the Saccharopolyspora species (ATCC No.98102), building a genom DNA library from the separated genom DNA by a conventional method, digesting the library DNA with SapI restriction endonuclease, again transforming the competent cells with the SapI restriction endonuclease and subsequently selecting a SapI-resistant clone.