ENZYME ACTIVITY MEASUREMENT

PROBLEM TO BE SOLVED: To detect and reject an abnormal value for attaining accurate measured results by sequentially-calculating an gradient between respective photometry points in reacting process to obtain respective relative values, and comparing them with preset permissible values for confirmation of the linearity of a change in an absorption value. SOLUTION: In this enzyme activity measurement, such as GOT, which permits enzyme to react in a container disposed on a rotating disc and obtains the enzyme activity by the change of absorbancy, absorbancy measuring is conducted at a prescribed number of photometry points of a reaction time course. Gradient (absorbancy variance per unit-hour) is computed sequentially from the photometry point lm where calculation preset as analysis parameter is started, through points lm+1 , lm+2 ,..., the point ln where the calculation is completed. A relative value is calculated by a prescribed method for respective calculated gradients, and comparative discrimination with permissible value preset arbitrarily is conducted. By rejecting the absorbancy at the point which is discriminated to be abnormal compared with the permissible value, and obtaining absorbancy changing speed by the method of least squares from remaining data, it is possible to calculate the enzyme activity.