OPTICAL SOLID-PHASE BIOSENSOR USING STREPT AVIDIN AND BIOTINAS BASE MATERIAL

PROBLEM TO BE SOLVED: To specifically recognize a specimen via the Forster energy shift from a specimen marker dye by binding a streptoavidin layer on the biotin layer of the uppermost layer of multiple layers of polyanion and polycation on a transparent base material, and binding a donor dye. SOLUTION: Contents of polyanion and polycation are alternately laminated on a transparent base material, the uppermost layer is made biotin polycation- containing layer, and the percentage of biotin is set to about 20-80mol% based on the equivalent number of the cationic group. Streptoavidin is bound to the uppermost layer to cover the laminated layers, and streptoavidin is marked with a fluorescent (donor) dye F1. When a specimen marked with a fluorescent (receptor) dye F2 exists in a sample solution, the fluorescence intensity of the receptor is increased from the unbound state and the fluorescence intensity of the donor is decreased as a result of the Forester energy shift after the specific binding to an immobilized antibody. When fluorescence is measured on both of them, the concentration of the specimen can be obtained.