DETECTION OF GENE

PROBLEM TO BE SOLVED: To simply and rapidly detect a gene after amplification by carrying out the hybridization between a single-stranded gene to be an object of measurement and a gene fragment, bonded to the solid phase and complementary to the gene and then reacting an intercalation reagent therew...

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Bibliographische Detailangaben
1. Verfasser:	FUKUDA SHIGEHIRO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To simply and rapidly detect a gene after amplification by carrying out the hybridization between a single-stranded gene to be an object of measurement and a gene fragment, bonded to the solid phase and complementary to the gene and then reacting an intercalation reagent therewith. SOLUTION: The hybridization between a single-stranded gene to be an object of measurement and a gene fragment, bonded to a latex particle uniform in particle diameter and complementary to the gene is carried out or a double- stranded gene to be the object of measurement is brought into contact with the solid phase bonded to a gene fragment having the complementation to at least either thereof to dissociate the double-stranded gene into a single- stranded one. The hybridization is then carried out on the solid phase to form double strands and an intercalation reagent containing a fluorescent coloring matter, etc., capable of entering the double-stranded gene and remarkably changing the fluorescent intensity or fluorescent wavelength is reacted therewith to simply and rapidly detect a gene after the amplification such as a human leukocytic antigen(HLA) typing.