OLIGONUCLEOTIDE CAPABLE OF DETERMINING SPECIES OF PASTEURELLA PISCICIDA

PROBLEM TO BE SOLVED: To obtain the subject new oligonucleotide, capable of determining the species, having a specific base sequence and useful as a reagent, etc., for identification capable of rapidly and simply identifying the species of a fish disease bacterium Pasteurella piscicida which is a pa...

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Bibliographische Detailangaben
Hauptverfasser:	AOKI CHU, KITAO TADATOSHI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To obtain the subject new oligonucleotide, capable of determining the species, having a specific base sequence and useful as a reagent, etc., for identification capable of rapidly and simply identifying the species of a fish disease bacterium Pasteurella piscicida which is a pathogenic bacterium of bacterial pseudotuberculosis constantly prevailing in a culture farm. SOLUTION: This new oligonucleotide is useful for the determination of the species of a fish disease bacterium Pasteurella piscicida comprising a base sequence represented by the formula and identification, etc., of the species of Pasteurella piscicida which is a pathogenic bacterium of bacterial pseudotuberculosis for fishes, constantly prevailing in a culture farm and causing a great economic loss. The oligonucleotide is obtained by selecting a base sequence specific for the Pasteurella piscicida from a gnomic DNA of the Pasteurella piscicida and carrying out the condensation and synthesis in the order of the sequence in the presence of a condensing agent by using a commercially available synthetic raw material corresponding to each of adenine, cytosine, thymine and guanine.