GENE DETECTION METHOD
PROBLEM TO BE SOLVED: To easily detect a gene after amplification by reacting a double-chain gene amplified by using a gene fraction connected to an antigen or an antibody to a particle connected to an antigen or an antibody for recognizing the antigen or the antibody, etc. SOLUTION: A double-chain...
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Zusammenfassung: | PROBLEM TO BE SOLVED: To easily detect a gene after amplification by reacting a double-chain gene amplified by using a gene fraction connected to an antigen or an antibody to a particle connected to an antigen or an antibody for recognizing the antigen or the antibody, etc. SOLUTION: A double-chain gene formed by amplifying a gene fraction connected to an antigen or an antibody is made to react with a particle connected to an antigen and an antibody for recognizing the antigen or the antibody, or a particle connected with a substance specifically connected to the antigen or the antibody. The targeted gene is detected by measuring cohesion rate of the particle. When a mutation part is presented on the gene used for amplification, restriction enzyme changing its cutting form by the presence of the mutation part is operated, so that the presence/nonpresence of the cutting is generated in the gene. The mutation of the gene is detected by detecting the subsequent change in cohesion rate. Familial amyotrophic lateral sclerosis, red blood cell enzyme defects, and hemophilia are included as detected mutations. |
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