GENE INTRODUCTION METHOD BY MICROBE OF GENUS AGROBACTERIUM AND PRODUCTION METHOD FOR TRANSFORMED PLANT

PROBLEM TO BE SOLVED: To efficiently introduce a gene to the plant of the genus Brassica and to produce transformed plants by pre-culturing the tissue piece of the plant belonging to the genus Brassica, then immersing it in infection liquid containing the microbes of the genus Agrobaterium and cultu...

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Bibliographische Detailangaben
1. Verfasser:	TAKASAKI TSUYOSHI
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To efficiently introduce a gene to the plant of the genus Brassica and to produce transformed plants by pre-culturing the tissue piece of the plant belonging to the genus Brassica, then immersing it in infection liquid containing the microbes of the genus Agrobaterium and culturing it under specified conditions. SOLUTION: After the tissue piece of the plant belonging to the genus Brassica such as Brassica rapa is pre-cultured in a tissue culture medium whose pH is 5.0-6.9, it is immersed in the infection liquid containing the microbe of the genus Agrobacterium, the immersed tissue piece is coexistingly cultured in the tissue culture medium and the gene is introduced to the plant of the genus Brassica. Also, it is preferable to provide a feeder cell in the tissue culture medium and it is preferable to set the values of X, Y and Z so as to satisfy the formula 82