METHOD FOR RECOVERING OXIDIZED FORM OF BETA-NICOTANAMIDE-ADENINE DINUCLEOTIDE

PURPOSE: To obtain an oxidized form of β-nicotinamide-adenine dinucleotide effectively in a high yield, useful as the reagent for the biochemical research, the determination factor of an enzyme analysis in the clinical diagnosis, etc., by contacting an acid of a prescribed concentration to the micro...

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Hauptverfasser: FUJII HIROSHI, MAEKAWA HIROKAZU, MIYA TOYOFUMI
Format: Patent
Sprache:eng
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Zusammenfassung:PURPOSE: To obtain an oxidized form of β-nicotinamide-adenine dinucleotide effectively in a high yield, useful as the reagent for the biochemical research, the determination factor of an enzyme analysis in the clinical diagnosis, etc., by contacting an acid of a prescribed concentration to the microbes of an yeast, and then extracting the microbes. CONSTITUTION: This method for recovering an oxidized form of β-nicotinamide- adenine dinucleotide comprises contacting 50-500mM acetic acid, formic acid, etc., with the microbes in which β-nicotinamide-adenine dinucleotide content is increased, of an yeast such as Candida utilis cultured in a medium enriched with nicotinamide, and then extracting the microbes.