GENE FRAGMENT, ETC., OF ANTIBODY RECOGNIZING MUCIN-TYPE SYNTHETIC GLYCOLIPID

PURPOSE: To obtain gene fragments coding for the V-regions of a H-chain and a L- chain of an antibody recognizing a mucin-type synthetic glycolipid, having specific base sequences and capable of efficiently producing an antibody against a cancer- specific sugar chain, which is useful as the treating agent, diagnostic, etc., of cancer. CONSTITUTION: Mouse is immunized with a mucin-type synthetic glycolipid (F1α antigen) and B-cells are collected from the mouse. The B-cell is fused with a mouse bone marrow cell to prepare F1α 75 hybridoma. An mRNA is extracted from the hybridoma and a cDNA is prepared from the mRNA by an ordinary method. Polymelase chain reaction(PCR) is carried out using a template of the obtained cDNA and a probe of each of a partial base sequence of the V-region of the H-chain and the V-region of the L-chain of the antibody. Thus, a DNA coding for the V-region of the H-chain and that of the L-chain of the antibody are separately multiplied to obtain the objective gene fragments coding for the V-region of H-chain of the antibody recognizing F1α and containing the base sequence of formula I, and that coding for the V-region of L-chain of the antibody recognizing F1α and containing the base sequence of formula II.