HEPTAPRENYLDIPHOSPHORIC ACID SYNTHETASE AND DNA CODING THE SAME

PURPOSE:To obtain a peptide useful for synthesizing heptaprenyldi-phosphoric acid(HDP) which is an intermediate for biosynthesis of prenylquinone, etc. CONSTITUTION:A peptide derived from Bacillus stearothermophilus has an amino sequence from 1st to 220th amino acids or an amino acid to or from whic...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	OGURA KYOZO, OBATA ATSUO, FURUYAMA TANETOSHI, KOIKE AYUMI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	PURPOSE:To obtain a peptide useful for synthesizing heptaprenyldi-phosphoric acid(HDP) which is an intermediate for biosynthesis of prenylquinone, etc. CONSTITUTION:A peptide derived from Bacillus stearothermophilus has an amino sequence from 1st to 220th amino acids or an amino acid to or from which one or several amino acids is substituted, lost or added. A transformant (pTL6/JM109, etc.) transformed by manifestation vector containing DNA coding the peptide is cultured and a peptide having heptaprenyldiphosphoric acid synthetase activity is obtained from culture products. Several gene fragments are obtained from PCR of a part of sequence of well-known prenyltransferase and gene region in the vicinity of these gene fragments is cloned and HDP synthetase gene derived from Bacillus stearothermophilus is cloned by measurement of manifested activity.