PRODUCTION OF HUCLEIC ACID-RELATED SUBSTANCE

PURPOSE: To obtain a recombinant DNA containing DNA carrying genetic information concerning synthesis of amidephosphoribosyltransferase of a microorganism belonging to the genus Corynebacterium and capable of producing a nucleic acid-related substance for seasoning, raw material, etc., for medicine...

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Hauptverfasser: FUJIO TATSURO, HAGIWARA TAKESHIGE, KATSUMATA RYOICHI
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creator FUJIO TATSURO
HAGIWARA TAKESHIGE
KATSUMATA RYOICHI
description PURPOSE: To obtain a recombinant DNA containing DNA carrying genetic information concerning synthesis of amidephosphoribosyltransferase of a microorganism belonging to the genus Corynebacterium and capable of producing a nucleic acid-related substance for seasoning, raw material, etc., for medicine raw materials. CONSTITUTION: This recombinant DNA contains a DNA fragment carrying genetic information concerning synthesis of amidephosphoribosyltransferase belonging to the genus Corynebacterium or Brevibacterium and a vector DNA. A microorganism belonging to the Corynebacterium or Brevibacterium is transformed using the recombinant DNA and the resultant transformant is cultured in a culture medium to produce a nucleic acid-related substance such as inosine, 5'-inosinic acid or 5'-xanthylic acid. in the cultured product. Thereby, a nucleic acid-related substance useful as a seasoning, medicine raw material, etc., can be obtained in high yield. The recombinant DNA is obtained by treating acidphosphoribosyl transferase gene with a restriction enzyme and then binding the treated gene to a vector.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title PRODUCTION OF HUCLEIC ACID-RELATED SUBSTANCE
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