PRODUCTION OF HUCLEIC ACID-RELATED SUBSTANCE

PURPOSE: To obtain a recombinant DNA containing DNA carrying genetic information concerning synthesis of amidephosphoribosyltransferase of a microorganism belonging to the genus Corynebacterium and capable of producing a nucleic acid-related substance for seasoning, raw material, etc., for medicine raw materials. CONSTITUTION: This recombinant DNA contains a DNA fragment carrying genetic information concerning synthesis of amidephosphoribosyltransferase belonging to the genus Corynebacterium or Brevibacterium and a vector DNA. A microorganism belonging to the Corynebacterium or Brevibacterium is transformed using the recombinant DNA and the resultant transformant is cultured in a culture medium to produce a nucleic acid-related substance such as inosine, 5'-inosinic acid or 5'-xanthylic acid. in the cultured product. Thereby, a nucleic acid-related substance useful as a seasoning, medicine raw material, etc., can be obtained in high yield. The recombinant DNA is obtained by treating acidphosphoribosyl transferase gene with a restriction enzyme and then binding the treated gene to a vector.