GENE DNA FOR DETERMINING PASTEURELLA PISCICIDA

PURPOSE: To obtain a new determination gene DNA having a specific restriction enzyme map and a mol.wt., used for identifying Pasteurella piscicida strain which is a bacterium causing bacterial nodositas, and enabling to simply and rapidly identify the bacterium causing fish infections disease. CONST...

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Hauptverfasser:	AOKI CHU, KITAO TADATOSHI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE: To obtain a new determination gene DNA having a specific restriction enzyme map and a mol.wt., used for identifying Pasteurella piscicida strain which is a bacterium causing bacterial nodositas, and enabling to simply and rapidly identify the bacterium causing fish infections disease. CONSTITUTION: This gene DNA has a DNA restriction enzyme map of the formula and a mol.wt. of approximately 0.8kbp, and is used for determining Pasteurella piscicida strain which is a bacterium causing bacterial nodositas. The gene DNA is useful as a reagent for identifying the bacterial kind of a fish disease, enabling to simply and rapidly identify a bacterium causative of an infectious disease due to the bacterium of the fish disease generated in a fish culture pond, etc. The DNA is obtained by culturing Pasteurella piscicida KG 8601 strain in a culture medium, collecting the cultured cells, extracting the chromosomal DNA from the collected cells by a conventional method, treating the extract with a restriction enzyme, ligating the obtained fragments to vectors, transducing the vectors into Escherichia coli, screening the Escherichia coli in the presence of a probe by a colony hybridization method, selecting a strain containing the strain-determining gene, and subsequently recovering the DNA.