METHOD FOR DISTINGUISHING PAPERMAKING SPOT USING NUCLEIC ACID AND PRIMER USED THEREFOR

PURPOSE:To directly detect the cause of a papermaking spot by taking advantage of chemical amplification and hybridization of DNA and to enable specification of the species of a microorganism existing in the spot which has been completely impossible. CONSTITUTION:A papermaking spot is distinguished by successively carrying out (A) a process for extracting a DNA from a a papermaking spot part, (B) a process for chemically amplifying the extracted DNA, (C) a process for isolating and purifying the amplified DNA, labeling the DNA and preparing a probe, (D) a process for culturing microorganisms existing in white water, isolating each microorganism, extracting a DNA from the microorganism and optionally amplifying and (E) a process for hybridizing the probe obtained by the process (C) and the DNA, subjected to electrophoresis, of each microorganism obtained by the process (D), respectively, and specifying a microorganism having the same sequence as that of the DNA of the microorganism contained in the spot.