GENE AMPLIFICATION METHOD

PURPOSE:To easily carry out the construction of a DNA library of the desired region of a chromosome, etc., by synthesizing various kinds of DNA sequences to cover an arbitrary region of a template DNA thermally denaturated in a reaction solution, and subjecting the DNA sequence to PCR amplification. CONSTITUTION:A single kind of oligonucleotide is annealed in an arbitrary region of a thermally denaturated template DNA for performing the PCR amplification of a DNA fragment having unknown base sequence such as a physically cut fragment of a desired region of a chromosome and a DNA sequence cloned as a YAC library, etc. The oligonucleotide is used as a PCR primer and a DNA chain complementary to a template DNA is extended therefrom to synthesize a complementary DNA corresponding to the arbitrary sequence region of the template DNA. The cycle of denaturation, annealing and extension is repeated to synthesize various kinds of DNA sequences covering an arbitrary region of the template DNA. Each of the DNA sequences is subjected to PCR amplification using the above oligonucleotide as a primer.