METHOD FOR MEASURING ALPHA-AMYLASE ACTIVITY AND REAGENT COMPOSITION THEREFOR

PURPOSE:To accurately measure alpha-amylase activity with a reagent containing a maltooligosaccharide binding a chromogen to reducing end glucose and binding a beta-galactose to a nonreducing end glucose, alpha-glycosidase and a beta-glycosidase inhibitor. CONSTITUTION:A reagent containing beta-gala...

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Hauptverfasser:	MAJIMA HATSUICHI, ASANO SHIGEKI, KIKUCHI TOSHIRO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE:To accurately measure alpha-amylase activity with a reagent containing a maltooligosaccharide binding a chromogen to reducing end glucose and binding a beta-galactose to a nonreducing end glucose, alpha-glycosidase and a beta-glycosidase inhibitor. CONSTITUTION:A reagent containing beta-galactosylmaltooligosaccharide (e.g. 4-nitrophenyl 4-O-beta-D-galactopyranosyl-alpha-maltotetraoxide) binding a hydroxy group at the first position of reducing end glucose to chromogen (e.g. p- nitrophenol) and modifying a hydroxy at 4 position or 6 position of nonreducing end glucose with beta-galactose, alpha-glucosidase and/or beta-glucosidase and a beta- galactosidase inhibitor (e.g. beta-D-thiogalactosyls) is reacted with alpha-amylase in a sample (e.g. pancreatic juice) and the produced chromogen is measured by spectrophotometer, etc. Thereby, action of j-galactosidase in a body fluid is suppressed and alpha-amylase activity is accurately measured.